Myogenin antibody (C-Term)

Catalog No. ABIN498068

The Goat Polyclonal anti-Myogenin antibody has been validated for WB and EIA. It is suitable to detect Myogenin in samples from Human, Mouse, Rat, Pig, Dog and Cow.

Quick Overview for Myogenin antibody (C-Term) (ABIN498068)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Myogenin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Myogenin Antibody

Binding Specificity: C-Term

Sequence: C-TDAHNLHSLT SIVD

Specificity: Reciognizes Myogenin/MYF4 (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus (near) of the protein sequence according to NP_002470.2. Genename: MYOG

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/16000. Western blot: 2-4 μg/mL. Detects a band of Approx 38 kDa in Mouse Skeletal Musclelysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: Myogenin

Background: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4

Molecular Weight: Human: 25.0kDa (NP_002470.2) Mouse: 25.2kDa (NP_112466.1).

Gene ID: 4656

NCBI Accession: NP_002470

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development